Aeromonas Aminopeptidase

Photochemical inactivation of Aeromonas aminopeptidase by 2,3-butanedione.

Isolation of the Leucine Aminopeptidase Gene from Aeromonas proteolytica

The leucine aminopeptidase of Aeromonas proteolytica (EC 3.4.11.10) is a monomeric metalloenzyme having the capacity to bind two Zn2+ atoms in the active site. Structural information of this relatively small aminopeptidase that could illuminate the catalytic mechanism of the metal ions is lacking; hence, we have obtained sequences from the purified enzyme, cloned the corresponding gene, and ex...

Aeromonas aminopeptidase. Improved isolation and some physical properties.

The extracellular aminopeptidase of Aeromonas proteofytica was purified to homogeneity by a simplified procedure that proved feasible for the isolation of gram quantities of this enzyme. Precipitation with ammonium sulfate and acetone, heating at 70”, and chromatography on Sephadex G-75 and DEAE-Sephadex yielded a product that showed a high degree of purity by polyacrylamide and free boundary e...

Isolation of the leucine aminopeptidase gene from Aeromonas proteolytica. Evidence for an enzyme precursor.

The leucine aminopeptidase of Aeromonas proteolytica (EC 3.4.11.10) is a monomeric metalloenzyme having the capacity to bind two Zn2+ atoms in the active site. Structural information of this relatively small aminopeptidase that could illuminate the catalytic mechanism of the metal ions is lacking; hence, we have obtained sequences from the purified enzyme, cloned the corresponding gene, and exp...

Heterologous expression and purification of Vibrio proteolyticus (Aeromonas proteolytica) aminopeptidase: a rapid protocol.

Metalloaminopeptidases (mAPs) are enzymes that are involved in HIV infectivity, tumor growth and metastasis, angiogenesis, and bacterial infection. Investigation of structure-function relationships in mAPs is a prerequisite to rational design of anti-mAP chemotherapeutics. The most intensively studied member of the biomedically important dinuclear mAPs is the prototypical secreted Vibrio proteo...